3/14/2024 0 Comments Identification ticks![]() Those annotated under the kingdom virus were initially identified as potential virus-associated sequences. After the adaptor sequences,low-quality bases and host genome were removed, resulting reads classified as a virus were subsequently de novo assembled into contigs, which were then compared to the NT database. The DNA library was constructed and then were sequenced with MGI High-throughput Sequencing Set (PE150). Total RNA was extracted and then enriched the viral RNA. Methods: Parasitic and host-seeking ticks were collected in Wolong Nature Reserve, Sichuan Province. JMTV has been confirmed to have a relatively wide distribution in vectors and hosts and is associated with human diseases. A class of multisegmented flavi-like viruses, Jingmen tick virus (JMTV) was first discovered in Rhipicephalus microplus ticks collected from Jingmen of Hubei Province, China in 2010. Introduction: Tick-borne viruses (TBVs) pose a significant risk to the health of humans and other vertebrates. 9College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.8BGI PathoGenesis Pharmaceutical Technology, BGI-Shenzhen, Shenzhen, China.7Academy of Inventory and Planning, National Forestry and Grassland Administration, Beijing, China.6Wolong National Natural Reserve Administration Bureau, Wenchuan, China.5Sichuan Forestry and Grassland Pest Control and Quarantine Station, Chengdu, China.4Beijing Macro & Micro-test Bio-Tech Co., Ltd., Beijing, China.3College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.2State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.1Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, China.Lin Huang 1,2 † Shunshuai Liu 2,3 † Lu Chen 4 Fei Wang 5 Ping Ye 6 Luoyuan Xia 2 Baogui Jiang 2 Hao Tang 6 Qingyu Zhang 6 Xiangdong Ruan 7 * Weijun Chen 8,9 * Jiafu Jiang 1,2 *
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